Place it as the final beat after a boast or failed argument, then follow it with Nelson's Ha-Ha for a longer mocking payoff, or open with Anime Punch before it to make the reply hit harder in the same scene.
About this sound
This short 2.6-second clip is Sakura Miko delivering the blunt English line 'Kiss my ass' in her bright, high-pitched voice, with the emphasis landing hard on the final word. It feels cheeky, defiant and deliberately rude in a playful way, like a cartoonish brush-off rather than a real insult. The line comes from Sakura Miko, the Hololive virtual YouTuber.