Place it at the end of a quiet buildup in a horror edit, then follow it immediately with the FNAF 2 Jumpscare Scream for a second hit, and finish with the Shocked Meme Sound as the on-screen reaction.
About this sound
This nine-second scary sound is a short, frightening clip made to catch a listener off guard. Its unsettling mood creates a tense, jump-scare feeling that works best when nobody expects it, turning a quiet moment into a quick jolt of fear.