Place it at the very end of a failed-run sequence, after the moment of realisation and before any reaction. Use It Was At This Moment He Knew to mark the turning point, then let this clip deliver the verdict, and follow it with a fail sound for the aftermath.
About this sound
This is a short Polish-language clip lasting about 3.3 seconds, built around the phrase Ślepa uliczka koniec gry, meaning a dead end and the end of the game. It lands as a blunt, final verdict after a failed attempt, with a dry, resigned humor that suits a run going nowhere. The wording makes the defeat feel complete rather than sudden.