Place this clip just before the scare hits, then follow it immediately with a FNAF jumpscare scream for the payoff. For a longer build, start with a danger alarm meme sound, let this laugh land second, and hold the scream until the reveal fills the screen.
About this sound
This is a short, sharp laugh for Sonic.EXE, the horror version of Sonic, delivered in a taunting, sinister tone over about two seconds. It feels unsettling and predatory, turning a familiar friendly character into something wrong in an instant. It comes from Sonic horror creepypasta and fangame culture that spread in the early 2010s.