Place it at the end of a reveal, after a Dramatic Dun Dun Dun builds the tension, then let a Vine Boom land just after it to punch the scare into the next scene.
About this sound
This is a short, roughly five-second eerie laugh tied to Sonic.EXE, the horror version of Sonic the Hedgehog, delivered in a cold, mocking voice that makes a familiar hero feel wrong. It feels creepy and unsettling, like something has gone wrong inside a game. It comes from creepypasta and fangame culture around the character, which spread online in the early 2010s through fan stories, games and videos.