Put it after the setup clip and before the reaction shot, with a punch sound first for the hit and a vine boom after for emphasis, so the warning closes the exchange and the next scene can start clean.
About this sound
This short voice clip, just under four seconds long, delivers a blunt threat about a kick in the ass, then pivots to asking to chill and relax. The sudden switch from aggressive to laid-back makes it feel cheeky and confrontational, like a joke warning meant to defuse tension rather than start a fight. It lands best as a playful put-down between friends.