Place it near the start of a suspense edit, while the follower is still unseen, then bring in an evil laugh once the follower steps into view. If the scene turns into a chase, follow that moment with a Metal Gear Solid alert to mark the sudden change in pace.
About this sound
This is a musical cue built around a tense, creeping melody that suggests someone is being followed. It keeps the mood uneasy and watchful, turning an ordinary scene into one that feels quietly threatening. As a suspense cue, it gives a listener the sense of danger closing in without any words to explain it.