Put it at the peak of a gameplay edit, after the Subway Surfers Bass Boosted build-up and before the Evil Laugh payoff, so it marks the turning point and the laugh closes the joke.
About this sound
This is a brief, high-pitched scream lasting just over a second, centred on a single drawn-out 'Aaaaaaaaaah' vocal burst. It hits with sudden shock, then turns funny once the surprise passes, making it feel like a prank scare rather than real danger.