Place it in the middle of a fail sequence, after No God Please No sets up the moment and before Roblox Oof marks the landing. Let the fall carry the scene, then cut straight to the reaction shot for the biggest laugh.
About this sound
This two-second clip captures Mario letting out a high, panicked yell as he tumbles downward in Super Mario 64. It feels instantly funny and frantic, turning a small slip into a cartoon disaster before the landing even happens. The voice comes from the 1996 Nintendo 64 platformer, where the same yell marked a missed jump or a long fall.