Place it right after the sibling moment in your edit, then follow with the Eww Brother Eww Sound Button for the reply, or close the sequence with the Damn Meme Sound Button to underline the reaction.
About this sound
The Alpha Sister is a short voice clip centred on a single sister callout, delivered as a bold, direct reaction line. It gives listeners a cheeky, confident feeling, like someone is being playfully called out in front of friends, which makes the moment feel teasing rather than serious and keeps the tone light and funny.