The line is from Portal 2, Valve's 2011 puzzle game, where the chapter itself is titled The Part Where He Kills You. GLaDOS says it as Wheatley, voiced by Stephen Merchant, seizes control and tries to kill Chell near the finale. The chapter title also became an achievement name, helping the quote stick in gaming culture. Players clipped the delivery for edits and streams, and it spread as a shorthand warning for any obvious ambush, scripted betrayal, or moment where a friendly guide stops being friendly.
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GLaDOS delivers her deadpan warning from Portal 2 in this short voice clip, announcing the part where he kills you, often used for dramatic betrayal moments.
This clip captures GLaDOS calmly saying hello before announcing that this is the part where he kills you, in the flat robotic tone that makes the threat sound routine. The line comes as Wheatley turns from bumbling helper to villain in Portal 2, and the contrast between her polite greeting and the sudden danger is what makes it land. It lasts only a couple of seconds, but the pause and delivery turn a simple warning into dark comedy, perfect for a reveal where someone realises the trap has already been set.