The line comes from The Rescuers Down Under, Disney's 1990 animated sequel set in the Australian outback. Villain Percival C. McLeach wants the golden eagle Marahute, while his goanna sidekick Joanna constantly hunts for eggs to eat, a gag borrowed from real goannas raiding nests. McLeach's warning protects the eggs he plans to use as bait rather than let Joanna spoil his trap. Decades later the isolated clip resurfaced on soundboards and in edits, where its specific wording became a reusable no, that is not yours reaction.