Place it at the very end of a defeat scene, after the action has finished, then follow it with the Re:Zero Return By Death cue to restart the moment, or the Womp Womp Fail cue if the joke is that the loss was deserved.
About this sound
This is a short, roughly three-second death scream from Tobias Fate, a single raw vocal outburst with no words, just the sound of a character going down in a dramatic moment. It feels sudden, over-the-top and darkly funny, the kind of exaggerated cry that turns a defeat into a punchline instead of a tragedy.