Put it near the start of a cat compilation as the opening gag, then follow it with a cat laugh for the payoff and a sad meow song when the joke turns sweet, saving an Eow cat meow for a final call-and-response between clips.
About this sound
The two-second clip is a cat vocalising in short, rising and falling notes that sound like a tiny off-key song, with each 'u ii a i' syllable landing as a separate beat. It feels silly and oddly catchy, the kind of absurd animal noise that makes people smile before they know why.