Put it at the exact restart point after a frozen or paused section, with Dun Dun Dun just before it to hold the tension, then follow it straight away with Mario Jump as the action picks up again.
About this sound
Unfreeze is an 8.4-second sound effect for the moment a pause ends and time starts again. It is built to mark that restart clearly, so whatever was paused can pick up exactly where it left off. It feels brisk and relieving, like a held breath finally released after waiting for the go-ahead.