Put it immediately after the on-screen loss, then follow it with Womp Womp Fail to underline the defeat before cutting to the next scene. For a gaming death that sends you back to the start, follow it with Re:Zero Respawn to signal the restart and keep the joke moving.
About this sound
This 8-second clip features VTuber Uruha Rushia crying out about her diamonds in a loud, high-pitched, distressed voice. It feels frantic and comically desperate, like a sudden loss hitting all at once. It comes from a moment when she left her microphone live, catching her raw, unguarded reaction as it happened.