This clip has no clear original source, and its appeal comes from that simplicity rather than from a famous scene or character. A long, wordless uhhhh is a universal reaction sound, so it circulates on soundboards and in short-form edits as a quick way to signal hesitation, doubt, or a brain freeze. Creators reuse it because it needs no context, fits almost any awkward setup, and is short enough to drop between lines of dialogue without breaking the pace of a video.