Place it immediately after the on-screen fail or reveal, before you cut back to commentary. For a bigger sequence, lead in with the FNAF Jumpscare Scream, follow with this reaction, then close with No God Please No if the moment keeps escalating.
About this sound
This is an 8-second Arabic voice clip of a man shouting Wa Boy Alaysh with no music behind him, just a high, stretched-out exclamation. It feels alarmed and comic at once, like genuine disbelief tipped into mock outrage, which is why listeners read it instantly as stunned surprise even without understanding the words.