Put it at the end of a rejection scene, right after the group walks away, then follow it with the SpongeBob Sad Song to underline the punchline before cutting to your reaction.
About this sound
This four-second voice clip is a small child asking, in a hurt and puzzled tone, why Chuckie does not want to play with him. The delivery is slow, soft and a little wobbly, like a kid trying not to sound upset while clearly feeling left out. It lands as sweet, sad and instantly relatable, turning a simple playground rejection into a moment that feels both funny and genuinely pitiful.