Place it right after the failed attempt in a short edit, then follow it with Roblox Oof to turn one miss into a longer running gag, keeping this clip as the middle beat before the next joke lands.
About this sound
This is the short 'ohh' vocal from the Nintendo party game, lasting about a second and a half, with a soft, rounded crowd-like groan. It feels gently disappointed and a little embarrassed, more playful than mocking, so a miss lands as a light joke rather than a harsh put-down. The sound comes from Nintendo's Wii Sports, released in 2006, where it played as a reaction to a failed attempt.