Place it right after a failed attempt or a rejected request, then follow it with Emotional Damage for a bigger reaction or Bruh when the refusal needs a flat, unimpressed finish.
About this sound
A Spanish voice repeats “ya no quiero” — “I don’t want to anymore” — over about six seconds, stretching the refusal out until it sounds exhausted rather than angry. The delivery feels fed up, defeated and stubborn at once, like someone who has reached a limit and will not be talked back into the task. It works as a comic sigh of surrender for anyone who recognises that exact moment.