Place it at the peak of a teasing exchange, just after the setup line lands and before the scene cuts away. Follow it with the Awkward Moment sound to let the joke settle, or pair it with the Anime Ahh sound for a louder second reaction from another character.
About this sound
This 2.5-second clip is a high-pitched anime-style voice crying out 'yamete kudasai' — Japanese for 'stop, please' — in a drawn-out, panicked ahh that stretches the plea into a squeal. It feels frantic, embarrassed and playfully desperate, like someone caught mid-mischief begging for mercy before things go any further.