Use it as the final beat of an edit, after the main story has finished and before the credits or closing image. Follow it with an outro sound for the finish, and keep it away from loud punchline clips so the closing moment stays clear.
About this sound
This eight-second clip gives the familiar 'You Are My Sunshine' tune a cowboy flavour, with the well-known loving line sung in a simple, western-style delivery. It feels warm, gentle and affectionate, like a small musical hug for someone dear, and it lands best as a brief, heartfelt moment rather than a loud joke.