The sound is tied to Jotaro Kujo, the central hero of JoJo's Bizarre Adventure: Stardust Crusaders, one of the most memed parts of Hirohiko Araki's long running manga and anime series. Short threatening lines from the show are widely clipped for edits because the series is known for dramatic poses, intense stares and over the top confrontations. Fans reuse this warning in fan edits, reaction posts and gaming videos whenever someone is singled out as the next opponent, keeping the moment alive beyond its original scene as a quick anime threat.