Place it at the very end of a fail sequence, after Enemy Spotted builds the danger and just before the screen cuts away, then follow it with Inception Horn to stretch the defeat into a closing beat for the edit.
About this sound
The clip is an eight-second dramatic game-over cue built around a low, swelling sting and the stark 'YOU DIED' moment from Dark Souls. It feels solemn, crushing and oddly funny, turning a small mistake into a grand, hopeless ending. It comes from Dark Souls, where it marks the end of a run after defeat.