Place it at the very end of a failed run, after the mistake is clear, and let it close the scene on its own. For a bigger game-over beat, play Roblox Oof first for the hit, then follow with this clip, or use FNAF Jumpscare Scream before it when the loss comes from a sudden scare.
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This is a short descending musical cue, lasting about three and a half seconds, with a light bouncy tune that tumbles downward instead of finishing on a strong note. It feels playful and gently comic, turning a loss into a small joke rather than a defeat. It comes from Nintendo's Super Mario games, where it plays when Mario loses a life.