Put it right after Enemy Spotted to announce the fight, then let it carry the entrance before cutting to No God Please No when the plan falls apart and the defeat needs a punchline.
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This is a short, under-a-second burst of the fast Megalovania melody, played on bright, driving chiptune-style notes with no voices. It feels tense, playful and suddenly serious, like a challenge has just been thrown down. The tune is Sans's boss theme from the game Undertale, where it plays during his final fight.