Place it immediately after a confusing statement or failed explanation, before the next scene cuts away, so the reaction carries the joke. Follow it with a dramatic sting for emphasis, or pair it first with a short alert sound and then this reaction for a two-step misunderstanding gag.
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This one-second clip catches Futurama's elderly inventor Professor Farnsworth asking 'Eh wha?' in a shaky, bewildered voice after missing what was just said. It feels instantly funny and a little endearing, like being caught out by a daft question you never saw coming. The line comes from the animated television series Futurama, where he often reacts to confusing news with the same muddled question.