Place it just after the last line in a waiting scene, then follow it with awkward crickets before the next scene begins. If the wait needs a bigger payoff, cut to a chipmunk laugh instead, keeping it as the setup and the second sound as the release.
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This 24-second clip is built around Squidward sitting on a chair, with the moment stretched out while he stays put. It gives an awkward, deadpan feeling, uncomfortable in a funny way, as if everyone is waiting for the next move. That patient, slightly tense mood makes it easy to recognise and easy to reuse.