There is no documented original source for this short 'não clique' clip, so it is best understood as an online reaction sound rather than a line from a named show or film. Its appeal comes from the clarity of the Portuguese warning, which is easy to grasp even for listeners who only recognise the phrase from memes and short videos. In Portuguese-language edits it works as an instant stop sign, while in wider meme culture it is often used ironically, because the person on screen ignores it.