This sound has no widely recognised film, television or meme origin, and it appears to have been shared simply as a labelled frequency tone. Short test tones like this are commonly uploaded to soundboards because they are easy to reuse, easy to recognise and useful across many kinds of edits. Its value comes from that versatility rather than from a famous scene, which is why creators tend to use it as a functional effect for alerts, errors and tension instead of as a joke or reference.