Place it at the end of a villain reveal, after the plan is shown, so it closes the scene. Follow it with Dun Dun Dun as the next beat, or open with Vine Boom before the reveal to set up the scene, then let this laugh finish the sequence.
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This 2.5-second clip is Adu Du's echoing evil laugh, a high, theatrical cackle that rises and repeats as if bouncing around a room. It feels gleefully menacing, like a cartoon villain savouring a plan that has just worked. It comes from BoBoiBoy, where Adu Du is the scheming alien antagonist.