No single widely documented source is attached to this brief clip, so it is best understood as a piece of online reaction audio rather than a line from a specific show or film. Short phrases like this travel easily on soundboards and in short-form video, where a familiar tone of mock disbelief can be recognised in a second. That portability is why it keeps appearing: it does not need setup, a character, or a backstory to make the joke land for a new audience.
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A short voice clip of someone saying now come on now in an exasperated tone, perfect for reacting to mistakes or unbelievable moments in videos and memes.
This is a very short spoken clip, just over a second long, in which a voice delivers the phrase now come on now with a drawn-out, teasing emphasis on the middle words. The delivery sounds half-amused and half-exasperated, like someone calling out a friend who has just said or done something obviously silly. Because it is so brief and instantly recognisable as a reaction, it lands as a quick punchline rather than a full line of dialogue, letting a viewer feel the speaker's disbelief without needing any extra explanation or context.