Place it at the peak of a chase or ambush, just as the threat appears on screen, then follow it with the FNAF Jumpscare Scream for the payoff and the Inception Horn Sound Effect to keep the scene going after the scare.
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This is the howl of the fast headcrab zombie from Half-Life 2, a three-second ragged shriek that climbs quickly before breaking into a strained, almost human wail. It feels panicked and aggressive, like something wounded is sprinting straight at you, and it puts listeners instantly on edge. Valve introduced the creature in Half-Life 2 in 2004, and the cry became familiar through ambushes in Ravenholm.