Place it at the peak of a chase or ambush sequence, just after an Enemy Spotted cue and before a FNAF Jumpscare Scream, so the encounter builds from warning to attack to full scare. It also works as a punctuation mark between fast cuts in a zombie montage.
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This is the short, under-a-second howl of a fast headcrab zombie from Half-Life 2, a sharp, animal-like cry from one of the game's quick, lunging creatures. It feels frantic and startling, like something has just spotted you and is already closing in. Taken from the Half-Life 2 creature sounds, it carries the game's tense, hostile atmosphere in a single burst.