Place this clip right after the mistake appears on screen, then follow it with the SpongeBob Sad Song Sound Button for the comedown. For a bigger fail sequence, lead in with the Windows XP Error Sound Button so the error hits first and this reaction closes the scene.
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This is a three-second voice clip of Steven stretching out a single desperate 'Noooooo!' in a high, panicked shout. It feels funny and overdramatic at the same time, turning a small disaster into a huge cartoon meltdown. The clip comes from the animated series Steven Universe, where Steven reacts with his whole heart to bad news.