Place this clip at the exact frame a mistake becomes visible, then follow it with the Windows XP Error Sound for a second hit, or precede it with the Law & Order Dun Dun to set up a mock-serious reveal before the reaction lands.
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This two-second clip is Will Forte as Andy delivering a drawn-out 'Oh no' that climbs in pitch and cracks as it stretches. It feels absurdly sincere and panicked, turning minor bad news into a comic meltdown. It comes from Saturday Night Live's Helping Hands Telethon sketch, first aired in season 30.