Place it at the interruption point itself, after normal dialogue and before the reaction shot. Follow it with the I'm Batman button for a hero response, or precede a villain reveal with the Imperial March button to build the entrance sequence.
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This is a short 2.5-second interruption sting associated with Bray Wyatt, built around a harsh, distorted burst that cuts in without warning. It gives listeners a creepy, unsettling feeling, like the room has suddenly turned hostile. The effect is known as the sound Bray Wyatt used to interrupt people on WWE television segments.