Place it at the exact moment a follower joins, before any welcome message, so it marks the arrival. Follow it with an Oh My God Meme reaction for a bigger surprise, then a Scream Meme if the nose gag escalates into chaos.
About this sound
This is a short German spoken alert lasting just over three seconds, built around the exclamation 'Ach du meine Nase' delivered in a playful, exaggerated tone. It feels funny and startled, like a cartoon reaction to a sudden smell or bump, and it works as a quick jolt of humour. It was made as a German Twitch alert, so it is designed to catch attention the moment a follower arrives.