Place it right after the reveal or mistake in your edit, as the sole reaction beat before cutting back to normal audio. Pair it after a record scratch sound for frozen disbelief, or before a crowd gasp sound when the moment needs to escalate.
About this sound
This is a short voice clip of television presenter Rodrigo Faro stretching out the exclamation "Ai mamãe" in a drawn-out, repeated delivery over about nine seconds. It gives a listener a feeling of playful panic and over-the-top dismay, turning a small shock or embarrassment into a big, funny reaction.