Place it as the closing beat of a short defeat edit, after the setup has made the failure clear. Open with It Was At This Moment He Knew to frame the mistake, then let this clip mark the shutdown, and follow with Fail Sound Meme to underline the flop for viewers who missed the first cue.
About this sound
This 1.2-second clip is the power-down sound for Albedo from Ben 10 Omniverse, a brief electronic shutdown that cuts the energy out from under him. It feels abrupt and deflating, like a villain losing his charge mid-scheme, with a dry comic edge that makes a sudden failure land harder. It comes from the animated series Ben 10 Omniverse.