Place it just after the defeat in a fail edit, as the setup before the reaction lands. Follow it with the Womp Womp Fail Sound to underline the loss, or cut to the Awkward Moment Sound Effect when the check-in interrupts the next scene.
About this sound
This 2.5-second clip is a single spoken question, 'Are ya winning, son?', delivered in the flat, slightly distant tone of a parent peering in from a doorway. It feels dryly funny and awkward, like well-meaning concern that completely misses what is actually happening on screen. It comes from the image-macro meme of a dad checking on his son at a computer.