The source recording for this two-second clip is not documented, so it is best understood as a standalone internet reaction sound rather than a line tied to one film or show. The phrase are you kidding me has long been used online as shorthand for disbelief, and short voice clips like this spread through memes, reaction videos and streams where creators need an instant, recognisable response. Like other reaction sounds such as the scream meme and the oh my god meme, it survives because it is short, clear and easy to drop over almost any surprising moment without explanation.