Place it immediately after the setup in a comedy edit, just before the visual payoff, then follow it with the fail sound when the gag collapses or with clapping when the joke lands and the crowd should cheer.
About this sound
This very short clip packs several vintage car horns into less than half a second, mixing classic awooga sounds with quick beeps and honks. It feels instantly silly and cartoonish, like a comic double-take when something absurd happens. The horns come from the 1949 Goofy cartoon Tennis Racquet, giving the burst its unmistakable old-Hollywood slapstick charm.