Place it immediately after the winning roast line, at the end of the exchange, then follow it with Danger Alarm Meme Sound to underline the defeat. For a longer reaction chain, open with No God Please No as the loser reacts, then let this clip deliver the final verdict.
About this sound
This roughly five-second voice clip repeats 'call an ambulance, call an ambulance' in a fast, shouting delivery, then flips the plea with the punchline that help is for the other person, not the speaker. It feels gleefully over the top, turning a sharp comeback into a mock emergency. The audio circulated as a short extract shared on Facebook as a reaction sound.