Place it at the end of an exchange, immediately after the offending line, so it acts as the final word. Follow it with Law and Order Dun Dun for a mock-serious verdict, or use Shut Up Mom next when the argument needs a second shut-down from another voice.
About this sound
This is a short Spanish voice clip, about 1.7 seconds long, in which a man snaps the line 'Cállese, hombre horrible' in a firm, annoyed tone. It feels blunt and funny, like a quick put-down that lands before anyone can argue back. The line comes from The Simpsons.