Place it at the peak of a fail or scare, just after the setup, then follow it with the Danger Alarm Meme Sound to extend the moment, or lead into it with the FNAF Jumpscare Scream before the reaction shot.
About this sound
Chuchel lets out a high, wailing scream that rises and holds like a siren for about five seconds, with no words, just a raw, stretched-out yell. It feels sudden, silly and over the top, the kind of noise that makes a quiet scene snap into chaos. The clip comes from the game Chuchel.