Place it at the exact frame of the loss, then follow it with an emotional damage meme sound if the reaction needs a second beat. For a drier punchline, pair it with the bruh sound effect instead, letting this clip carry the reaction while the other closes the scene.
About this sound
This is the King's crying emote audio from Clash Royale, a very short burst of cartoony, exaggerated sobbing lasting just over a second. It feels playfully pathetic rather than genuinely sad, turning a small defeat into theatrical mock grief. The clip comes from Supercell's mobile game Clash Royale, where the King emote is instantly recognised by players.