Place it at the peak of an awkward reveal, after Sudden Suspense builds the tension and before Brother Eww extends the reaction, then cut straight to the next scene while viewers are still reacting.
About this sound
This is an ultra-short anime-style reaction clip, lasting under a second, with a high, girlish voice that sounds instantly uncomfortable and suspicious. It gives a quick burst of secondhand embarrassment, funny because the awkwardness hits so suddenly, and it works as an immediate cue that something feels off.