Place it right after a long explanation in your edit, just before the reaction shot, then follow it with Ceeday Huh when the listener still does not respond, and save Sudden Suspense for the moment the other person still says nothing.
About this sound
A voice credited to AJ repeats the question "Did I lose you, did I?" across about thirteen seconds, checking in with a listener who seems to have drifted away. The delivery feels uncertain and a little pleading, like someone waiting for an answer that has not come yet, which makes it land as a gentle nudge rather than a scolding.